32. Reis DQP, Calvário J, Chibeles I, Kumar M, Pina, AS*. Optimizing (Bio) Catalysis with Liquid-Liquid Phase Separation Systems. ChemSystemsChem e00089 (2025).
31. Calvário J, Antunes D, Cipriano R, Kalafatovic D, Mauša G, Pina, AS*. Understanding Biases in Liquid–Liquid Phase Separation: Investigating Amino Acid Enrichments in Phase-Separating Proteins toward Peptide Design. Biomacromolecules (2025).
30. Reis DQP, Pereira S, Ramos AP, Pereira PM, Morgado L, Calvário J, Henriques AO, Serrano M, Pina AS*. Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity. Nat Commun 15, 9368 (2024).
29. Janković P, Babić M, Perčić M, Pina AS, Kalafatovic D*. Factors influencing the catalytic activity of metal-dependent histidine-rich peptides: sequence, conformation, stereochemistry, self-assembly or their interplay? Mol Syst Des Eng 8 (11), 1371-1380 (2023).
28. Dias AMFC, Moreira IP, Lychko I, Nurrito A, Barbosa AJM, Lutz-Bueno V, Mezzenga R, Carvalho AL, Soares C, Pina AS, Roque ACA. Hierarchical self-assembly of the reflectin-derived protopeptide. Front Chem 11, 1 – 14 (2023).
27. Pina AS*, Morgado L, Duncan KL, Carvalho S, Carvalho HF, Moreira IP, Kalatatovic D, Barbosa AJM, Mariz BP, Fautino, BMM, Pappas C, Wang T, Narang V, Ferreira I, Roque ACA*, Ulijn RV*. Discovery of phosphototyrosine-binding oligopeptide with supramolecular target selectivity. Chem Sci 13, 210-217 (2022).
26. Carvalho S, Reis DQP, Pereira SV, Kalafatovic D, Pina AS*. Catalytic peptides assemblies: challenges between simplicity and functionality. Isr J Chem 62, e202200029 Invitation for the Special Issue Peptide and Protein Self-Assembly and Interactions (2022).
25. Matos MJB, Trovão F, Gonçalves J, Rothbauer U, Freire MG, Barbosa AMJB, Pina AS, Roque A.C.A. A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent. Sep Purif Technol 265, 118476 (2021).
24. Janković P, Pina AS, Kalafatović D*. Exploiting peptide self-assembly for the development of minimalistic viral mimetic. Front Chem 23473. Invitation for the Special Issue International Women of Supramolecular Chemistry (2021).
23. Mariz BP, Carvalho S, Batalha IL, Pina AS*. Artificial enzymes bringing together computational design and directed evolution. Org Biomol Chem 2021, 19, 1915 – 1925 (2021).
22. Pappas GC*, Wijerathne N, Sahoo JK, Jain A, Kroiss D, Sasselli IR, Pina AS, Lampel A, Ulijn RV*. Spontaneous Aminolytic Cyclization and Self- Assembly of Dipeptide Methyl Esters in Water. ChemSystemsChem 2 (2020).
21. Fernandes CSM, Pina AS, Roque ACA*. Affinity-triggered hydrogels: Developments and prospects in biomaterials science. Biomaterials, 268, 120563-120563 (2020).
20. Fernandes CSM, Rodrigues AL, Alves V, Fernandes TG, Pina AS*, Roque ACA*. Natural multimerization rules the performance of affinity-based physical hydrogels. Biomacromolecules, 21, 8, 3081–3091 (2020).
19. Padrão I, Fernandes CSM, Esteves C, Fernandes TG, Pina AS*, Roque ACA*. Versatile and Tunable Poly (Ethylene Glycol)‐ Based Hydrogels Crosslinked through the Ugi Reaction. ChemPlusChem 85, 2732-2741 (2020).
18. Roque ACA*, Pina AS, Azevedo AM, Heng J, Haigh J, Ottens M, Aires-Barros R. Anything but conventional chromatography approaches in Bioseparation. Biotechnol J, e1900274:1-8 (2020).
17. Matos MJB, Pina AS, Roque ACA*. Rational design of affinity ligands for bioseparation. J Chromatogr A (invited), 1619: 460871 (2020).
16. Fernandes, C, Pina AS, Barbosa AJM, Padrão I, Duarte F, Andreia C, Teixeira S, Alves V, Gomes P, Fernandes TG, Dias AMGC, Roque ACA*. Affinity‐triggered assemblies based on a designed peptide‐peptide affinity pair. Biotechnol J14, 1800559 (2019).
15. Dos Santos R, Figueiredo C, Viecinski AC, Pina AS, Barbosa AJM, Roque ACA*. Designed affinity ligands to capture human serum albumin. J Chromatogr A 1583, 88-97 (2018).
14. Hussain A, Semeano ATS, Palma SICJ, Pina AS, Almeida J, Medrado BF, Pádua ACCS, Carvalho AL, Dionísio M, Li RWC, Gamboa H, Ulijn RV, Gruber J, Roque ACA*. Tunable Gas Sensing Gels by Cooperative Assembly. Adv Funct Mat 1700803, 1–9 (2017).
13. Fernandes CSM, Gonçalves B, Margarida M, Martins DL, Barroso T, Pina AS, Peixoto C, Aguiar Ricardo AI, Roque ACA*. Biobased Monoliths for Adenovirus Purification. ACS Appl Mater Interfaces 7, 6605¬6612 (2015).
12. Fernandes CSM, Pina AS, Batalha IL, Roque ACA*. Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic affinity ligands. Sep Sci Technol 52: 2909-2917 (2017).
11. Pina AS, Carvalho S, Dias AMGC, Guilherme M, Pereira AS, Caraça LT, Coroadinha AS, Lowe CR, Roque ACA*. Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins. J Chromatogr A, 1472: 55-65 (2016).
10. Fernandes CSM, Barbosa I, Castro R, Pina AS, Coroadinha AS, Barbas A, Roque ACA. Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage‐display. Biotechnol J 11, 1-12 (2016).
9. Pina AS*, Dias AMGC, Ustok FI, El Khoury G, Fernandes CSM, Branco, RJF, Lowe CR, Roque ACA. Mild and cost-effective Green Fluorescent Protein employing small synthetic ligands. J. Chromatogr. A 1418, 83-93 (2015).
8. Pina AS*, Batalha IL, Fernandes CSM, Aoki MA and Roque ACA*. Exploring the potential of magnetic antimicrobial agents for water disinfection. Water Res. 66, 160¬168 (2014).
7. Pina AS, Guilherme M, Pereira AS, Fernandes CSM, Branco RJF, Lowe CR, Roque ACA*. A tailor made affinity pair “tag-receptor” for the purification of fusion proteins. ChemBiochem 15(10):1423-1435 (2014).
6. Pina AS, Lowe CR, Roque ACA*. Challenges and opportunities in the purification of recombinant tagged proteins. Biotechnol Adv 32(2): 366-381 (2014).
5. Fernandes CSM, Pina AS, Dias AMGC, Branco R, Roque ACA*. A theoretical and experimental approach towards the development of affinity adsorbents for GFP and GFP ¬fusion proteins purification. J Biotechnol 186, 13-20 (2014).
4. Santana, SDF, Pina AS, Roque ACA*. Immobilization of enterokinase on magnetic supports for the cleavage of fusion proteins. J Biotechnol 161: 378-382 (2012).
3. Pina AS, Lowe, Roque ACA*. Comparison of fluorescence labelling techniques for the selection of affinity ligands from solid-phase combinatorial libraries. Sep Sci Technol 45, 2187–2193 (2010).
2. Hussain A, Pina AS, Roque ACA*. Bio-recognition and detection using liquid crystals. Biosensors and Bioelectronics 25: 1-8 (2009).
1. Pina AS and Roque ACA*. Interaction of potent bioactive peptides with angiotensin converting enzyme. Journal of Molecular Recognition 22, 162-168 (2009).
Book Chapters
2. Pina AS, Batalha IL, Roque ACA, “Affinity Tags in Protein Purification and Peptide Enrichment”, in Protein Downstream Processing, Methods in Molecular Biology 1129, 147-168 USA: Humana Press Inc. (2014)
1. Pina AS, Hussain A. and Roque ACA, An historical overview of drug discovery”, in Ligand-Macromolecule Interactions in Drug Discovery (ed. Roque, A.C.A.), Methods in Molecular Biology 572, 3-12, USA: Humana Press Inc. (2009)
Bioinspired Peptide Systems Lab 